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Food Science and Technology International
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Prototype Instruments for Laboratory and On-Line Measurement of Lipoxygenase Activity

J. I. Reyes De Corcuera

Department of Biological Systems Engineering, Washington State University, Pullman WA 99164-6120 USA

R. P. Cavalieri

Agricultural Research Center, Washington State University, Pullman WA 99164-6240 USA

J. R. Powers

Department of Food Science and Human Nutrition, Washington State University, Pullman WA 99164-6376 USA

A laboratory bench-top and a continuous prototype for measurement of lipoxygenase activity was developed. Lipoxygenase activity determination was based on the measurement of the rate of oxygen consumption by the enzyme using a polarographic sensor for the bench-top prototype and fibre optic sensors for the continuous prototype. The bench-top and the continuous sensors were compared against the standard spectrophotometric method. The range of LOX activity according to the spectrophotometric assay was from 0 to 0.163 µmol/L s. Accuracy and precision were 0.009 and 0.005 µmol/L s respectively for the bench top prototype and 0.007 and 0.006 µmol/L s for the on-line prototype. Correlation coefficients (R 2) were 0.99 for both prototypes with respect to the spectrophotometric method.

Key Words: lipoxygenase activity • blanching • bench-top prototype • on-line sensor prototype

Food Science and Technology International, Vol. 9, No. 1, 5-9 (2003)
DOI: 10.1177/1082013203009001001
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