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Food Science and Technology International
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Modeling the Influence of pH, Temperature and Culture Medium Composition on the Kinetics of Growth and Cysteine Proteinase Production by Micrococcus sp. INIA 528 in Batch Culture

A. F. Mohedano

J. A. Casas

Area de Ingeniería Química, Facultad de Ciencias, Universidad Autónoma, 28049 Madrid, Spain

J. Fernández

Departamento de Tecnología de Alimentos, INIA, Carretera de La Coruña Km. 7, 28040 Madrid, Spain

F. García-Ochoa

Departamento de Ingeniería Química, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain

M. Nuñez

Departamento de Tecnología de Alimentos, INIA, Carretera de La Coruña Km. 7, 28040 Madrid, Spainnunez{at}inia.es

Kinetics of biomass and cysteine proteinase formation during growth of Micrococcussp. INIA 528 in batch cultivation were studied. Modeling of cultivation data was carried out using an unstructured model. The logistic equation was found to be adequate to describe microbial growth. Production of cysteine proteinase was described by means of an equation including biomass and substrate concentrations. An overall model based on an equation of substrate consumption dependent on biomass formation and proteinase production was postulated. The kinetic parameters (specific growth rate, maximum biomass, proteinase production rate and proteinase formation yield) were obtained taking into account the influence of pH (6.0–8.0), temperature (28–41 ºC) and the addition of different supplements to the basal culture medium [yeast extract, MgSO 4,glucose, K2HPO4 and (NH4)2SO4]. Kinetic parameters were calculated by fitting experimental data by a nonlinear technique using the algorithm of Marquardt coupled to a fourth-order Runge-Kutta subroutine.

Key Words: modeling • kinetics • Micrococcus • proteinase production • batch cultivation

Food Science and Technology International, Vol. 7, No. 1, 49-57 (2001)
DOI: 10.1106/12N7-G4VD-27JR-JQLT
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