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Food Science and Technology International
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Lipase-catalyzed esterification of glycerol with n-3 polyunsaturated fatty acids from winterized fish oil Esterificación catalítica de glicerol con ácidos grasos 3-n poliinsaturados de aceite de pescado

V.J. Robles

Instituto Tecnológico de Veracruz, Departamento de Ing, Quimica y Bioquímica, Apdo, Postal 420 Veracruz, Ver. 91860, México

H.S. García

Instituto Tecnológico de Veracruz, Departamento de Ing, Quimica y Bioquímica, Apdo, Postal 420 Veracruz, Ver. 91860, México

J.A. Monroy

Instituto Tecnológico de Veracruz, Departamento de Ing, Quimica y Bioquímica, Apdo, Postal 420 Veracruz, Ver. 91860, México

O. Angulo

Instituto Tecnológico de Veracruz, Departamento de Ing, Quimica y Bioquímica, Apdo, Postal 420 Veracruz, Ver. 91860, México

Menhaden oil was hydrolyzed using a lipase from Pseudomonas sp. The hydrolysate was cold frac tionated at-72°C. Glyceride synthesis was performed using the same lipase under different reaction environments. The best conditions for the esterification reaction were 39 °C for 18 h in a reaction mixture containing anhydrous glycerol, n-3 polyunsaturated fatty acids (PUFA) enriched solution (2% lipids in hexane), hexane, and phosphate buffer-lipase solution (1% w/v). Product composition was 81.33% triacylglycerides and 18.67% of free fatty acids (w/w). Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) accounted for 36.18% of the esterified fatty acids, of which 58% was EPA and 42% was DHA. This method offers an alternative to produce glycerides rich in n-3 PUFA.

Key Words: n-3 polyunsaturated fatty acids • PUFA • lipases • fish oil • transesterification • winterization

Food Science and Technology International, Vol. 4, No. 6, 401-405 (1998)
DOI: 10.1177/108201329800400604
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