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Anisakis Simplex Antigens in Fresh and Frozen-thawed Muscle of Anchovies in Vinegar

Cellular Biology Department, Faculty of Biology, UCM, Madrid, Spain

M.L. García

Centro de Microscopía Electrónica "Luis Bru", UCM, Madrid, Spain

C. de las Heras

Instituto del Frío, Consejo Superior de Investigaciones Científicas (CSIC) Madrid, Spain

A.I. Rodriguez-Mahillo

Immunology Department, Hospital Carlos , Madrid, Spain

M. Gonzalez-Munoz

Immunology Department, Hospital Carlos , Madrid, Spain

I. Moneo

Immunology Department, Hospital Carlos , Madrid, Spain

A. Mendizábal

Technical Unit of Mercamadrid, Department of Food Safety, Public Health Institute, Madrid-Salud Municipality Department of Safety and Community Services, Council of Madrid, Madrid, Spain

M. Tejada

Instituto del Frío, Consejo Superior de Investigaciones Científicas (CSIC) Madrid, Spain, mtejada{at}if.csic.es

Marinated fish treatment using low pH to enlarge the storage life of fish as in anchovies in vinegar, does not kill Anisakis simplex larvae infesting fish muscle. To kill the larvae it is compulsory in many countries to freeze fish intended to be marinated raw, which prevents the consumer to be infested with the live larvae. However, it is not known if A. simplex antigens are released to the media after freezing and vinegar processing, which may cause allergic reaction to A. simplex sensitized consumers. Anchovy fillets were artificially infested with A. simplex L3, treated with a vinegar solution and chilled stored for 10 days. Infested frozen-thawed fillets were treated and stored in the same conditions. Viability of the larvae, SEM, immunoblotting and immunohistochemistry were performed on the treated fillets before and after pepsin treatment. Viability of the larvae was detected only in the chilled fillets; however, A. simplex antigens were detected in the chilled and in the frozen-thawed fillets even after pepsin treatment. This suggests that the consumption of anchovies in vinegar may be a potential hazard when ingested by sensitized consumers, even if freezing kills the larvae.

Key Words: Anisakis simplex • antigens • anchovies • vinegar • food hygiene

Food Science and Technology International, Vol. 15, No. 2, 139-148 (2009)
DOI: 10.1177/1082013208105171


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