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Food Science and Technology International
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Acyl Transferase Domains of Putative Polyketide Synthase (PKS) Genes in Aspergillus and Penicillium Producers of Ochratoxin A and the Evaluation of PCR Primers to Amplify PKS Sequences in Black Aspergillus Species

P.V. Martínez Culebras

Departamento de Medicina Preventiva y Salud Pública, Ciencias de la Alimentación, Bromatología, Toxicología y Medicina Legal, Universitat de València. Vicente Andrès Estellès s/n 46100 Burjassot, Valencia, Spain, pmartinez{at}iata.csic.es, Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC). PO Box 73, 46100 Burjassot, Valencia, Spain

A. Crespo-Sempere

Departamento de Medicina Preventiva y Salud Pública, Ciencias de la Alimentación, Bromatología, Toxicología y Medicina Legal, Universitat de València. Vicente Andrès Estellès s/n 46100 Burjassot, Valencia, Spain, Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC). PO Box 73, 46100 Burjassot, Valencia, Spain

J.V. Gil

Departamento de Medicina Preventiva y Salud Pública, Ciencias de la Alimentación, Bromatología, Toxicología y Medicina Legal, Universitat de València. Vicente Andrès Estellès s/n 46100 Burjassot, Valencia, Spain, Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC). PO Box 73, 46100 Burjassot, Valencia, Spain

D. Ramón

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC). PO Box 73, 46100 Burjassot, Valencia, Spain

Fungal polyketide synthases (PKS) are responsible for the biosynthesis of several mycotoxins and other secondary metabolites. PKS genes in ochratoxin producing species from Aspergillus and Penicillum genera have been identified using a degenerate primer pair developed for the acyl transferase (AT) domain of fungal PKSs. Sequences of AT domains were aligned and analyzed using phylogenetic methods. The AT domain sequences appeared to be specific for a particular type of fungal PKSs and were related to PKSs involved in different mycotoxin biosynthesis pathways, including ochratoxin A. We have also developed primers suitable for amplifying AT domain sequences in strains belonging to the A. niger aggregate. DNA from most of the black Aspergillus species currently recognized was tested. Primers showed general applicability and other Aspergillus species belonging to section Nigri were successfully amplified.

Key Words: Aspergillus • Penicillium • mycotoxin • ochratoxin A • polyketide synthase • PCR

Food Science and Technology International, Vol. 15, No. 1, 97-105 (2009)
DOI: 10.1177/1082013208102743
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