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Changes in Gene and Protein Expression during Tomato Ripening — Consequences for the Safety Assessment of New Crop Plant Varieties

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands, esther.kok{at}wur.nl

S.J. Lehesranta

Institute of Applied Biotechnology, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland

J.P. van Dijk

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands

J.R. Helsdingen

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands

W.T.P. Dijksma

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands

A.M.A. Van Hoef

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands

K.M. Koistinen

Institute of Applied Biotechnology, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland

S.O. Karenlampi

Institute of Applied Biotechnology, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland

H.A. Kuiper

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands

J. Keijer

RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands

An important part of the comparative approach to assess the safety of new crop plant varieties is an extensive compositional analysis, including the measurement of all key nutrients and antinutrients in a specific crop. The study described here investigates the applicability of `omics' technologies, transcriptomics and proteomics, as additional tools in this comparative safety assessment. The aim of the work was to assess the extent of the natural variation in ripening tomato fruits as a model crop and to determine whether it is possible to develop simple `ripening stage' criteria for the sampling of fruits for `omics' analyses. It is shown that the set-up of an `omics' study is of crucial importance. Samples under scrutiny should be well-matched with relation to environmental conditions during growth and harvest, including the stage of ripening, as is stipulated in international guidance documents for the nutritional and toxicological assessment of genetically modified plants.

Key Words: transcriptomics • proteomics • safety assessment • tomato ripening • GMO

Food Science and Technology International, Vol. 14, No. 6, 503-518 (2008)
DOI: 10.1177/1082013208100771


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