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Food Science and Technology International
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Inter-laboratory Trial to Validate the Direct Solvent Extraction Method for the Identification of 2-dodecylcyclobutanone in Irradiated Chicken and Whole Liquid Egg

I. Tewfik

School of Biosciences, University of Westminster, 115, New Cavendish Street W1W 6UW, London, United Kingdom, I.Tewfik{at}westminster.ac.uk

The main objective of this interlaboratory trial was to assess the qualitative performance of new rapid direct solvent extraction (DSE) method as potential detection method to differentiate between irradiated and unirradiated food samples. The trial was designed and carried out between four European laboratories that agreed to use 2-dodecylcyclobutanone (DCB) as radiolytic marker to detect irradiated minced chicken and liquid whole egg. Chicken samples were irradiated at 3 and 7 kGys while egg samples were irradiated at 3 and 6 kGys. Every laboratory had received 12-blind coded `unknown' and 4-known coded samples to be analyzed. All four laboratories were able to correctly identify all 12-blind coded samples as either irradiated (at medium and/or high doses) or unirradiated samples. The tested DSE method is regarded as fast, cheap, suitable and recommended for use in laboratories which are involved in screening large number of food samples for irradiation.

Key Words: inter-laboratory trial • validation • direct solvent extraction method • 2-dodecylcyclobutanone • irradiated chicken

Food Science and Technology International, Vol. 14, No. 3, 277-283 (2008)
DOI: 10.1177/1082013208095689
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