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Food Science and Technology International, Vol. 14, No. 2, 151-156 (2008)
DOI: 10.1177/1082013208092560

Note. Genetic Diversity of Food Isolates of Salmonella enterica serovar Typhimurium in India

S.D. Saroj

Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

R. Shashidhar

Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

J.R. Bandekar

Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India, jrb{at}barc.gov.in

The study aimed to determine the genetic diversity of Salmonella enterica serovar Typhimurium isolates from sprouts and fish samples from India. Twenty-eight S. Typhimurium isolates were genotyped by pulsed-field gel electrophoresis and tested for antibiotic susceptibility, plasmid profile, and presence of virulence genes (invA and spvC). Three distinct XbaI-pulsed-field profiles (PFPs) were observed. Out of 22 isolates from sprouts, 20 showed PFP-A and remaining two showed PFP-B. All six fish isolates showed PFP-C. All the strains were sensitive to the examined antibiotics except two strains of PFP-A. Two different plasmid profiles P1 and P2 were observed. P2 was observed only in the antibiotic resistant strains. All strains were positive for the invA, whereas spvC was detected only in sprout isolates. There was no specific pattern in occurrence of the genotypes with respect to seasons. However, PFP-A was dominant and was isolated throughout the year. This is the first report to assess the genotypic diversity of S. Typhimurium obtained in foods from India. As S. Typhimurium is the predominant serotype in India, the data generated in this study would be useful in epidemiological studies during outbreaks of Salmonellosis in India.

Key Words: PFGE • molecular characterization • genotyping • Salmonella


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