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Food Science and Technology International, Vol. 13, No. 5, 355-359 (2007)
DOI: 10.1177/1082013207085689

Isolation and Purification of an Anticoagulant from Schizymenia dubyi by Fermentation

P.M. Ekanayake

Department of Marine Biotechnology, Cheju National University, 66, Jejudaehakno Jeju 690-756, Republic of Korea

C. Nikapitiya

Department of Marine Biotechnology, Cheju National University, 66, Jejudaehakno Jeju 690-756, Republic of Korea

M. De Zoysa

Department of Marine Biotechnology, Cheju National University, 66, Jejudaehakno Jeju 690-756, Republic of Korea

Y.J. Jeon

Department of Marine Biotechnology, Cheju National University, 66, Jejudaehakno Jeju 690-756, Republic of Korea

J. Lee

Department of Marine Biotechnology, Cheju National University, 66, Jejudaehakno Jeju 690-756, Republic of Korea, jehee{at}cheju.ac.kr

Natural fermentation is a method of extracting anticoagulant compounds from red algae Schizymenia dubyi. Preliminary screening was carried out on the basis of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) assays. The optimal fermentation time period for the highest APTT, PT, and TT values was found to be 8 weeks. Purification of the fermented sample by DEAE-anion exchange chromatography followed by Sepharose-4B chromatography resulted in a single polysaccharide, which was reconfirmed by a single spot on agarose gel electrophoresis. The purified sample had >1000s APTT activity at 130 µg/mL. The molecular weight estimated by polyaccrylamide gel electrophoresis was >500,000 Da. This is the first report indicating the presence of the anticoagulant compound in S. dubyi.

Key Words: Schizymenia dubyi • anticoagulants • fermentation • red algae • APTT


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