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Food Science and Technology International
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Chemical Composition and Lipid Fraction Characteristics of Aleppo Pine (Pinus halepensis Mill.) Seeds Cultivated in Tunisia

S. Cheikh-Rouhou

Unité Analyses Alimentaires, Ecole Nationale d’Ingénieurs de Sfax, Route de Soukra 3038 Sfax, Tunisia

B. Hentati

Unité de Biotechnologie et Pathologie, Institut Supérieur de Biotechnologie de Sfax, Route de Soukra 3038 Sfax, Tunisia

S. Besbes

Unité Analyses Alimentaires, Ecole Nationale d’Ingénieurs de Sfax, Route de Soukra 3038 Sfax, Tunisia, besbes.s{at}voila.fr

C. Blecker

Unité de Technologie des Industries Agro-alimentaires, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgium

C. Deroanne

Unité de Technologie des Industries Agro-alimentaires, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgium

H. Attia

Unité Analyses Alimentaires, Ecole Nationale d’Ingénieurs de Sfax, Route de Soukra 3038 Sfax, Tunisia

The proximate composition of Aleppo pine (Pinus halepensis Mill.) seeds cultivated in Bizerta (Tunisia) were investigated for their fatty acid composition, and their thermal and physical properties of lipid fraction. The proximate analysis of pine seeds showed the following composition (on a dry-weight basis): protein 22.7%, oil 43.3%, ash 8.3% and total carbohydrate 25.7%. Potassium, magnesium and calcium were the predominant mineral elements present in the seeds and reached, together, about 1%. Oleic and linoleic acids were the major unsaturated fatty acids (27.3 and 48.8%, respectively), while the main saturated one was palmitic acid (8.75%). Myristic, myristoleic, palmitoleic, margaric, margaroleic, stearic, linolenic, arachidic, eicosenoic, eicosadienoic, eicosatrienoic, behenic and lignoceric acids were also detected. Thermal profile of Pinus halepensis Mill. seed oil, determined by its DSC melting curve, showed that the entire liquefaction of the Aleppo pine seed oil occurs at 8°C. CieLab colour parameters (L*, a*, b*), oxidative stability by Rancimat test and viscosity were also determined. Physico-chemical properties of the oil include: saponification number 190, peroxide value 3.18, iodine index 117, and a low acidity of 0.61%. Results suggested that the production of oil from Pinus halepensis seeds could provide a potential use in food, pharmaceutical, cosmetics and other non-food industries.

Key Words: Pinus halepensis Mill. seeds • oil • fatty acids • thermal profile

Food Science and Technology International, Vol. 12, No. 5, 407-415 (2006)
DOI: 10.1177/1082013206069910


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