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Food Science and Technology International, Vol. 12, No. 5, 371-377 (2006)
DOI: 10.1177/1082013206070410

Bioactive Composition of Pig Laryngeal Cartilage Extracts and Their Free Radical-scavenging Activity

Shuang-li Xiong

School of Food Science and Technology, Southern Yangtze University, 214036, Wuxi, Jiangsu Province, China

Zheng-yu Jin

School of Food Science and Technology, Southern Yangtze University, 214036, Wuxi, Jiangsu Province, China, zjin{at}stu.edu.cn

An-lin Li

School of Food Science and Technology, Southern Yangtze University, 214036, Wuxi, Jiangsu Province, China

This work focused on amino acid and mineral element analysis of pig laryngeal cartilage. Papain and DEAE-Sepharose fast flow ion exchange chromatography were used to hydrolyase cartilage and purify hydrolysate. Further study was performed to compare the free radical-scavenging activity of various fractions. Cartilage is abundant in bioactive amino acids such as glycine, glutamic acid and arginine and minerals such as calcium, magnesium and iron. Four polysaccahrides attached peptides and one peptide were isolated from hydrolysate. Weak 2,2-diphenyl-1-picryhydrazyl free radical scavenging activities of all substances were observed. Other fractions showed hydroxyl free radical and superoxide anion free radical scavenging ability in a concentration-dependent manner except fraction A. The hydroxyl free radical scavenging activity of tested samples decreased in the following order: fraction C crude chondroitin sulphate (ChS) peptide fraction B semi-purified ChS purified ChS. Significant differences were observed between purified ChS and other fractions (P 0.01). For superoxide anion free radical the order was: peptide purified ChS semi-purified ChS crude ChS fraction C fraction B. The peptide was significantly higher than purified ChS (P 0.01), which was significantly higher than the other components (P 0.01). According to the results, polysaccharides, ChS, amino acid and peptide present in cartilage had important bioactivity.

Key Words: cartilage • peptides • isolation • purification • radicals • scavenging


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