This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Laparra, J. M. Articles by Farré, R. Search for Related Content Social Bookmarking                         What's this?

Effect of Enzyme Amounts Used in Gastrointestinal Digestion Upon Solubility and Caco-2 Cell Uptake Assays of Minerals from Infant Formulas

Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 - Burjassot (Valencia), Spain

R. Barberá

Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 - Burjassot (Valencia), Spain, reyes.barbera{at}uv.es

R. Farré

Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 - Burjassot (Valencia), Spain

The effect of enzyme amounts used in gastrointestinal in vitro digestion upon the solubility and Caco-2 cell uptake of calcium, iron and zinc from infant formulas (IFs) was studied. Different amounts of enzymes (g enzyme/g IF), pepsin (0.002 and 0.048), pancreatin (0.0005, 0.002 and 0.01) and bile extract (0.003, 0.125 and 0.0625) were assayed. Mineral soluble contents and mineral uptakes by Caco-2 cells were affected by the enzyme amounts used in digestion. Although the highest mineral solubility (Ca 98.6 vs 46.2%; Fe 98.1 vs 83.9%; Zn 98.4 vs 83%) was obtained when the lowest enzyme (pepsin 0.002 vs 0.048; pancreatin 0.0005 vs 0.01g/g IF) and bile extract (0.003 vs 0.0625g/g IF) amounts were used, under these conditions uptake decreased with respect to that obtained when the highest amounts were used (Ca 0.4 vs 1.8%; Fe 0.3 vs 4.8%; Zn 1.7 vs 37%), that indicated a lack of relationship between solubility and absorption. Interactions among different products resulting from the proteolytic activity of digestive enzymes and minerals can modify the physicochemical forms in which minerals are available for absorption after gastrointestinal digestion.

Key Words: in vitro digestion • infant formula • minerals • uptake • Caco-2 cells

Food Science and Technology International, Vol. 11, No. 6, 425-431 (2005)
DOI: 10.1177/1082013205060195


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?