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Food Science and Technology International, Vol. 11, No. 4, 251-259 (2005)
DOI: 10.1177/1082013205056402

Protective Effect of a Lactobacillus salivarius Strain of Human Origin

A. I. Haza

A. Zabala

N. Arranz

Departamento de NutriciÛn, BromatologÌa y TecnologÌa de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

P. Morales

Departamento de NutriciÛn, BromatologÌa y TecnologÌa de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spainpmorales{at}vet.ucm.es

The protective effect of a Lactobacillus salivariusstrain from human faeces (HA8) was evaluated against the cytotoxicity of N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR), N-nitrosodibutylamine (NDBA) and N-nitrosopiperidine (NPIP) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. L. salivariusHA8 strain showed a moderate protective effect against NPYR and weak against NDBA and NPIP. No protective effect against cytotoxicity of NDMA was observed at the bacterial population used. To test the effect of L. salivariusHA8 on cytokine production (interleukin-1 ± (IL-1 ±), interleukin-8 (IL-8) and tumour necrosis factor alpha (TNF-≥)), the human macrophage cell line (THP-1) was cultured in the presence and absence of lipopolysaccharide (LPS). L. salivariusHA8 induced IL-1 ±, IL-8 and TNF-≥ releases when cells were stimulating with and without LPS.

Key Words: N-nitrosamines • cytotoxicity • L. salivarius • cytokines


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