Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Food Science and Technology International
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Bunkova, R.
Right arrow Articles by Lojek, A.
Right arrow Search for Related Content
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Analysis of Plant Extracts Antimutagenicity Using the Ames Test and the Cytogenetic Analysis of Peripheral Blood Lymphocytes

R. Bunkova

Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic, Regional Hygienic Station Brno, Genetic Toxicology. Pellicova 29/31, 602 00 Brno, Czech Republic

I. Marova

Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic, marova{at}fch.vutbr.cz

Z. Pokorna

Regional Hygienic Station Brno, Genetic Toxicology. Pellicova 29/31, 602 00 Brno, Czech Republic

A. Lojek

Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 00 Brno, Czech Republic

This work was focused on comparison of antimutagenic effect of selected plant extracts (carrot, paprika, tomato, spinach, onion, kiwi and green tea) analysed by two independent tests of genotoxicity: i) the Ames test with Salmonella typhimurium TA98 and ii) cytogenetic analysis of peripheral blood lymphocytes (CAPL). Both methods were proved without and/or with metabolic activation. Samples were allowed to be positive antimutagens based on their ability to inhibit the mutagenic effects of standard mutagens. High positive results (more than 60% of inhibition of mutagenic effect) were obtained with extract from green tea. Extracts from paprika, carrot and spinach as well as solutions of standard alltrans-carotene and some standard flavonoids showed antimutagenic (40-60% of inhibition) or weakly antimutagenic (20-40% of inhibition) effects. Positive antimutagenic effects of extracts from spinach and green tea were determined using CAPL method too. Further, the comparison of antimutagenic activity and antioxidant capacity of tested samples analysed using TRAP (total radical-trapping antioxidant parameter) method was proved. Extract from green tea showed the highest antioxidant as well as antimutagenic capacity, but no correlation was found between these parameters in all tested extracts.

Key Words: plant extracts • antimutagenicity • antioxidant capacity • Ames test • cytogenetic analysis

Food Science and Technology International, Vol. 11, No. 2, 107-112 (2005)
DOI: 10.1177/1082013205052764
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?