This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Web of Science (1) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Mizani, M. Articles by Khodabandeh, M. Search for Related Content Social Bookmarking                         What's this?

An Effective Method for Producing a Nutritive Protein Extract Powder from Shrimp-head Waste

Departmentof Food Science and Technology, Science and Research Campus, Azad University, P.O. Box 14155-4933, Tehran, Iran mizanil_2000{at}yahoo.com

M. Aminlari

Departmentof Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz 71345, Iran

M. Khodabandeh

Biological Product Group, National Research Center for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran

Enzymatic hydrolysis has been widely applied for production of protein hydrolysate from shrimp waste and for purification of chitin. In the present study, shrimp (P. semisulcatus) head waste was hydrolysed, using a commercial proteolytic enzyme, Alcalase. In order to improve protein extraction efficiency, certain chemicals such as sodium sulphite and Triton x-100 were used along with the enzyme. When Alcalase (12 AU/kg) used alone, the yield of protein extraction was 45.1% and by using Triton x-100 (0.01 g/kg) together with Alcalase, the yield was decreased to 39%, whereas the presence of sodium sulphite (200 mmol/L) with the enzyme or with the enzyme and Triton x-100 increased the level of protein extraction to 62% and 65.1%, respectively. The resulting protein powder contained sufficient amounts of essential amino acids to be used in feed formulations. By precipitating proteins from the resulting protein extract at pH 3.1, the residual sulphite in protein powder was decreased by 97% and thus the powder can be considered suitable for animal and/or aquaculture feed formulations.

Key Words: shrimp-head waste • P. semisulcatus • Alcalase • proteins • hydrolysis

Food Science and Technology International, Vol. 11, No. 1, 49-54 (2005)
DOI: 10.1177/1082013205051271


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?