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Food Science and Technology International
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A Novel Genetic Label for Detection of Specific Probiotic Lactic Acid Bacteria

L. Fernandez

M. L. Marin

S. Langa

R. Martin

C. Reviriego

A. Fernandez

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain

M. Olivares

J. Xaus

Departamento de Immunología, Puleva Biotech, 18004 Granada, Spain

J. M. Rodriguez

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spainjmrodrig{at}vet.ucm.es

A novel method for genetic labelling of specific lactic acid bacteria strains was developed. The approach implied the transformation of the hosts with a plasmid containing a heterologous DNA fragment. The sequence of a DNA fragment that has been used to label a variety of genetically modified (GM) soya was used to design a forward primer and three reverse primers yielding PCR products recognisable by their sizes. Stability of the recombinant plasmid in the transformed strains was studied by PCR, and the results varied significantly depending on the strain. To test the usefulness of the DNA label to study in vivo properties of probiotic bacteria, such as viability after transit through the digestive tract, mice were orally inoculated with a genetically-labelled Enterococcus faecium strain. Later, their faeces were aseptically collected and the genetically-labelled strain was detected among the colonies that grew on MRS agar.

Key Words: lactic acid bacteria • lactobacilli • probiotics • genetic labelling

Food Science and Technology International, Vol. 10, No. 2, 101-108 (2004)
DOI: 10.1177/1082013204043761
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